SDHB with Interpretation by Immunohistochemistry

Also known as: SDHB IP

Clinical Use

Use

The test screens for potential succinate dehydrogenase (SDH) germline mutations in patients with specific tumor types. Abnormal immunohistochemical staining for succinate dehydrogenase B (SDHB) correlates with the presence of a germline mutation in the genes of the succinate dehydrogenase complex, namely SDHB, SDHC, and SDHD, although it is not definitive. Genetic counseling is advised for further investigation when abnormal results are found. More comprehensive genetic testing is suggested to confirm findings and assess other genes associated with abnormal staining, such as SDHAF2 and SDHA.

Special Instructions

Not provided.

Limitations

This test has been developed and validated by ARUP Laboratories but it has not been cleared or approved by the FDA. Testing results must be correlated with clinical findings and patient history for accurate interpretation. The test is performed in a CLIA certified laboratory and is intended for clinical purposes. Mutations in genes other than those specifically targeted (SDHB, SDHC, SDHD) may also affect the results, indicating the need for comprehensive genetic assessment.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

SDHB

Protein

IHC

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Result Codes

94736-6 - Tissue block ID Spec
31208-2 - Specimen source
55229-9 - Immune stain study

Result Turnaround Time

1-5 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

Paraffin block or positively charged slides

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect from excessive heat and use ARUP Supply #47808 for transportation.