Toll-Like Receptor Function
Also known as: TLR
Use
This test aids in the diagnosis of innate immunodeficiencies when genetic defects of the innate immune system are suspected. It is particularly useful for individuals who are negative for other immunodeficiencies such as abnormalities in antibody function, complement activity, neutrophil function, or cell-mediated immunity. However, it does not measure the function of toll-like receptor 3 (TLR3).
Special Instructions
For patients who are lymphopenic, it is recommended to collect 10 mL of whole blood, rather than the minimum required volumes, to increase the likelihood of isolating a sufficient number of lymphocytes. The collection of a control specimen from a healthy individual unrelated to the patient is recommended for accurate interpretation.
Limitations
The test does not measure TLR3 function directly. Molecular testing is preferred for detecting defects in TLR3. The function of toll-like receptors is compromised in cases with defects in IRAK-4 and MyD88, except for endosomal TLR4, which operates independently of these pathways. Abnormal patient results without a client submitted control sample should be clinically correlated and interpreted cautiously.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Result Turnaround Time
9-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
7 mL
Container
Green (sodium heparin) tube or Yellow (ACD solution A) tube
Collection Instructions
Collect patient and control specimens under similar conditions; ensure collection within 48 hours of test performance.
Patient Preparation
Collect control specimen from a healthy individual unrelated to the patient.
Storage Instructions
Transport at room temperature; do not refrigerate or freeze.
Causes for Rejection
Yellow (ACD solution B), refrigerated or frozen specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |