Urticaria-Induced Basophil Activation
Also known as: UIBA
Use
The Urticaria-Induced Basophil Activation test is used to determine whether CD203c is upregulated in suspected chronic urticaria cases, particularly when associated with autoimmune antibodies to the basophil IgE receptor or to IgE. This aids in evaluating the presence of basophil stimulating antibodies, which can indicate an autoimmune contribution to chronic urticaria. The analysis assists clinicians in understanding the immunological mechanisms of chronic urticaria and to tailor patient management strategies based on the autoimmune status of the disease.
Special Instructions
Specimens must be serum and should be separated from cells as soon as possible, preferably within 2 hours of collection. The serum should be transferred to an ARUP Standard Transport Tube, with a minimum requirement of 0.5 mL. The sample must be kept frozen; it remains stable for 1 year if frozen immediately, but is unstable when refrigerated.
Limitations
The test has not been cleared or approved by the FDA. Results are dependent on the correct processing and transportation of specimens, as serum not frozen immediately may yield inaccurate results. It is crucial to avoid repeated freeze/thaw cycles to maintain sample integrity. The test is not suitable for samples other than serum, such as those that are contaminated, hemolyzed, or lipemic. Negative results do not entirely rule out the autoimmune etiology of urticaria, and clinical correlation is recommended.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 63369-3
Result Turnaround Time
7-16 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection and transfer to ARUP Standard Transport Tube.
Storage Instructions
CRITICAL FROZEN. Avoid repeated freeze/thaw cycles.
Causes for Rejection
Specimens other than serum. Contaminated, grossly hemolyzed, or lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 30 minutes |
| Refrigerated | Unacceptable |
| Frozen | 1 year |