Amylase Isoenzymes
Also known as: Amylase Fractionation, Isoamylase, Pancreatic Amylase, Salivary Amylase
Use
Evaluate parotitis and pancreatic disease. Amylase is primarily produced in the pancreas and salivary glands. Isoenzymes may be used to determine the source of an elevated amylase concentration. Measurement of pancreatic amylase activity is of value in diagnosing pancreatitis and other pancreatic disorders that result in the elevation of serum and urine amylase.
Special Instructions
For accurate results, it is important to separate serum or plasma from cells within 30 minutes of collection. Use the correct anticoagulants as other anticoagulants like citrate and fluoride inhibit the assay reaction.
Limitations
Patients with macroamylase may have elevated pancreatic amylase results that are not diagnostic for pancreatitis. Macroamylase forms, which are high molecular weight complexes, are too large for renal clearance and may increase plasma amylase activity significantly. This condition may be detected during investigations of abdominal pain.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 24333-7
- 1798-8
- 1805-1
- 1809-3
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
1 mL
Container
Red-top tube, gel-barrier tube, or green-top (heparin) tube
Collection Instructions
Separate serum or plasma from cells within 30 minutes of collection.
Storage Instructions
Room temperature; stable for seven days. Stable refrigerated for one month.
Causes for Rejection
Hemolysis; use of anticoagulants other than lithium heparin (Citrate and fluoride inhibit the assay reaction.)
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 1 month |