CD4:CD8 Ratio Profile

Clinical Use

Use

The CD4:CD8 Ratio Profile is an important test for assessing immune function, particularly in patients with HIV-1 infection. HIV-1 infection leads to a decrease in CD4 T cells, an increase in CD8 T cells, and a reduction in the CD4:CD8 ratio. This test provides prognostic information and helps monitor disease progression and antiretroviral therapy in HIV-1 seropositive patients by enumerating CD4 T cells.

Special Instructions

Not provided.

Limitations

This test's accuracy and reliability depend on adhering to specimen collection and handling protocols. Specimens must arrive at the laboratory within 48 hours to preserve cell viability. Hemolyzed, clotted, contaminated, or improperly stored specimens will be rejected. Specimens refrigerated or frozen are also unsuitable for this assay, as they affect the immunophenotyping process. The test results should be interpreted in the context of clinical and other laboratory findings.

Test Details

Methodology

Cell-based / Cytometry (Flow Cytometry)

Biomarkers

CD4 and CD8

Protein

% CD4 Pos. Lymph.

Expression • Quantitative (Continuous) (e.g., copies/mL, % expression)

Abs. CD4 Helper

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

% CD8 Pos. Lymph.

Expression • Quantitative (Continuous) (e.g., copies/mL, % expression)

Abs. CD8 Suppressor

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

CD4/CD8 Ratio

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Order Codes

65758-5 - T-cell CD4 subset pnl Bld

Result Codes

24467-3 - CD3+CD4+ Cells # Bld
8123-2 - CD3+CD4+ Cells NFr Bld
14135-8 - CD3+CD8+ Cells # Bld
8101-8 - CD3+CD8+ Cells NFr Bld
54218-3 - CD3+CD4+ Cells/CD3+CD8+ Cll Bld
6690-2 - WBC # Bld Auto
789-8 - RBC # Bld Auto
718-7 - Hgb Bld-mCnc
4544-3 - Hct VFr Bld Auto
787-2 - MCV RBC Auto
785-6 - MCH RBC Qn Auto
786-4 - MCHC RBC Auto-EntMCnc
788-0 - Erythrocyte DistWidth Bld Auto
777-3 - Platelet # Bld Auto
770-8 - Neutrophils NFr Bld Auto
736-9 - Lymphocytes NFr Bld Auto
5905-5 - Monocytes NFr Bld Auto
713-8 - Eosinophil NFr Bld Auto
706-2 - Basophils NFr Bld Auto
75652-8 - Imm Lymphs Bld Ql
751-8 - Neutrophils # Bld Auto
731-0 - Lymphocytes # Bld Auto
742-7 - Monocytes # Bld Auto
711-2 - Eosinophil # Bld Auto
704-7 - Basophils # Bld Auto
71695-1 - Imm Granulocytes NFr Bld Auto
53115-2 - Imm Granulocytes # Bld Auto
58413-6 - nRBC Bld Auto-Rto
18314-5 - Morphology Bld-Imp

Result Turnaround Time

1-3 days

Specimen

Whole Blood

Volume

Fill tube(s) to capacity.

Minimum Volume

Not provided

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection Instructions

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.