Chromosome Analysis, Leukemia/Lymphoma With Reflex to Chromosome Microarray (CMA)
Also known as: Hematologic Disorders, Chromosome Analysis, Philadelphia Chromosome
Use
Karyotyping, physical localization of copy number changes, and mapping of breakpoints involved in translocations for the diagnosis/prognosis of leukemia/lymphoma subgroups. The SNP assay will detect chromosomal imbalance that may be present in neoplastic disorders and clonal evolution.It provides detection of copy-neutral loss of heterozygosity of any chromosome, and the percent and location of homozygosity, that may be associated with cancer gene mutations.
Special Instructions
Indicate the tentative diagnosis on the test request form for proper culturing and result interpretation. Use the Leukemia/Lymphoma Specimen Transport Kit for bone marrow or peripheral blood and the Lymph Node Transport Kit for lymph node, spleen, or bone core. Specimens should arrive at the laboratory within 48 hours of collection. Patient should not be on chemotherapy.
Limitations
The SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes containing only heterochromatin, or tetraploidy. For myeloid malignancy studies, sodium heparin amounts, lithium heparin, or EDTA can inhibit cell culture growth. A normal karyotype does not rule out clonal molecular alterations below light microscopy resolution, and additional molecular or FISH testing may be warranted.
Methodology
Chromosomal / Cytogenetics (N/A)
Biomarkers
LOINC Codes
- 31208-2
- 31208-2
- 62361-1
- 62360-3
- 55199-4
- 62358-7
- 62356-1
- 62365-2
- 48672-0
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 mL
Minimum Volume
Not provided
Container
pediatric green-stopper (sodium heparin) tube
Collection Instructions
Submit at room temperature using the Leukemia/Lymphoma Specimen Transport Kit. Indicate date and time of collection on the test request form.
Patient Preparation
Patient should not be on chemotherapy.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Hemolysis; gross contamination; nonneoplastic cells; clotted specimen; frozen specimen; fixed specimen; improper or excessive anticoagulant.