Eosinophil Cationic Protein (ECP)
Also known as: Ribonuclease 3
Use
Measurement of the level of Eosinophil Cationic Protein (ECP) in serum
Special Instructions
Custom care should be taken during specimen collection. BD plastic serum-separator tubes should not be used as they yield erroneous results. Allow the sample to stand for 60 to 120 minutes for ECP release prior to centrifugation. Transfer the serum to a plastic transport tube post-separation. Refrigerate the serum after separation.
Limitations
The performance of this test has not been cleared or approved by the FDA. ECP's diagnostic utility is limited as its levels can be increased in other atopic and non-atopic diseases beyond asthma, such as allergic rhinitis and bacterial sinusitis. Its application is restricted to allergic-type asthma, and its correlation with lung function parameters is less reliable, especially in children with mild or moderate asthma. Non-serum samples, non-Greiner tube collections, and grossly hemolyzed or lipemic samples are causes for rejection.
Methodology
Immunoassay (ImmunoCAP®)
Biomarkers
LOINC Codes
- 25638-8
- 25638-8
Result Turnaround Time
4-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
NMR lipoprofile serum-separator tube (No. 60360)
Collection Instructions
Allow the sample to stand for 60 to 120 minutes to release the ECP in the cells prior to centrifugation. Separate the serum after 60 to 120 minutes and transfer to a plastic transport tube.
Storage Instructions
Refrigerate.
Causes for Rejection
Nonserum sample received; non-Greiner tube collected; grossly hemolyzed or lipemic samples
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 5 days |
| Refrigerated | 14 days |
| Frozen | 14 days |