Factor IX Antigen
Use
Measurement of factor IX antigen concentration
Special Instructions
Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. Mix the sample immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. Use a discard tube when using a winged blood collection device. Following centrifugation steps must be followed to prepare the plasma sample for testing.
Limitations
The Factor IX Antigen test has some limitations, including potential variability in results due to improper sample collection or handling. The accuracy of the test depends on the correct blood to anticoagulant ratio. Additionally, the test does not differentiate between functional and non-functional factor IX antigen, nor does it detect other coagulation abnormalities or diseases.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 3190-6
- 3190-6
Result Turnaround Time
4-8 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
1 mL
Container
Blue-top (sodium citrate) tube
Collection Instructions
Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Mix the sample immediately by gentle inversion at least six times. A discard tube is necessary when using a winged blood collection device. Freeze immediately and maintain frozen until tested.
Patient Preparation
Do not draw from an arm with a heparin lock or heparinized catheter.
Storage Instructions
Freeze. Stable at room temperature for eight hours.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 hours |