Factor V Leiden With Reflex to R2
Use
Detection of the factor V Leiden mutation, followed by testing for the factor V R2 polymorphism in individuals found to be positive for factor V Leiden (heterozygous). These tests provide information about increased risk for venous thrombosis, which can be helpful for prevention and for genetic counseling regarding risk to relatives. The factor V R2 polymorphism does not occur in individuals who are factor V Leiden homozygotes.
Special Instructions
The test includes the detection of Factor V Leiden and reflex testing for factor V R2 in cases of positive factor V Leiden results. It is important to maintain the specimen at the correct temperature post-collection to ensure the accuracy of the test results.
Limitations
The test does not detect all possible genetic mutations associated with thrombosis risk. Only the factor V Leiden and R2 polymorphisms are analyzed. Results may be influenced by the quality of the DNA extracted from the specimen, and clotted samples where adequate DNA cannot be obtained will be rejected. The test is not suitable for frozen blood samples.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 21667-1
- 49549-9
- 51968-6
- 77202-0
Result Turnaround Time
5-12 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube (preferred) or yellow-top (ACD) tube
Storage Instructions
Maintain specimen at room temperature or refrigerate. Stable at room temperature for eight days.
Causes for Rejection
Clotted whole blood samples if adequate DNA not obtained; incorrect specimen type; frozen whole blood specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 days |