Glutamic Acid Decarboxylase (GAD) Autoantibody
Also known as: Anti-GAD, GAD-65 Antibody
Use
This test is intended for the semiquantitative determination of glutamic acid decarboxylase (GAD) antibody in human serum; it is useful as an aid in the diagnosis of type 1 diabetes mellitus (autoimmune mediated diabetes).1
Special Instructions
This test may exhibit interference if the sample is collected from a person consuming high doses of biotin. Patients should stop biotin consumption at least 72 hours before sample collection to avoid interference with test results.
Limitations
The test may not be reliable in the presence of high doses of biotin, which can cause interference. Hemolysis and lipemic serum samples could also render the test inconclusive. Additionally, while it provides crucial predictive information for IDDM, it should be used in conjunction with other clinical evaluations and tests for comprehensive diagnosis.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 56540-8
- 56540-8
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.4 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
If a red-top tube is used, transfer separated serum to a plastic transport tube. Avoid hemolysis.
Patient Preparation
Patients should stop biotin consumption at least 72 hours prior to sample collection.
Storage Instructions
Room temperature storage is acceptable for up to 14 days.
Causes for Rejection
Nonserum sample; grossly hemolyzed or grossly lipemic sample received
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |