Gynecologic Pap Smear, One Slide
Use
The Gynecologic Pap Smear is utilized for diagnosing primary or metastatic neoplasms and genital infections caused by various pathogens including herpesvirus, Candida, Trichomonas vaginalis, cytomegalovirus, Actinomyces, and human papillomavirus (HPV). It also assists in evaluating hormonal function through maturation index in vaginal specimens.
Special Instructions
Include patient's name, date of birth, Social Security number (or other ID), history of malignancy, drug/radiation therapy, last menstrual period (LMP), menopausal status, surgical history, hormone use, abnormal bleeding, and any other relevant clinical details on the test request form. Pap tests may be sent for pathologist review if atypical or malignant cell changes are suspected.
Limitations
The test may be limited by inadequate collection of cell populations from ectocervical, endocervical, or vaginal sites. Use of lubricating jelly or presence of starch granules can interfere with cytological examination, leading to unsatisfactory results. The test is not sufficient to detect a significant proportion of endometrial adenocarcinomas and might miss some endocervical adenocarcinomas and aggressive cervical squamous lesions.
Methodology
Immunoassay (Other)
Biomarkers
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Container
Slides and other supplies, including cytospray fixative and slide holders, available from courier service.
Collection Instructions
Preferred fixative: Cytospray fixative or 95% ethyl alcohol. Label slides with patient name and site of origin using a graphite pencil. Prepare endocervical smears by rotating brush in endocervical canal; ectocervical smears by scraping the entire ectocervix; vaginal smears using spatula on the posterior fornix. Fix each slide immediately with spray fixative or 95% ethyl alcohol.
Patient Preparation
Avoid douches 48 to 72 hours before the exam. Do not collect specimen during or shortly after menstrual period. Minimize use of lubricating jelly.
Storage Instructions
Maintain specimens at room temperature. Spray-fixed slides can be stored indefinitely.
Causes for Rejection
Improper labeling, fixation in formalin, air-drying artifact