Hepatitis C Virus (HCV) Antibody Cascade to Quantitative PCR and Genotyping
Use
Diagnosis and management of hepatitis C virus (HCV) infection. A positive antibody test will reflex to a quantitative PCR assay. When the quantitative viral load is >1000 IU/ml an additional reflex to genotyping will occur.
Special Instructions
Not provided.
Limitations
The test may exhibit interference when a sample is collected from a person who is consuming a supplement with a high dose of biotin. Additionally, results may vary in accuracy in immunocompromised individuals where early or acute infection might delay detection.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 40726-2 - HCV IgG SerPl Ql IA
- 13955-0 - HCV Ab SerPl Ql IA
- 11011-4 - HCV RNA SerPl NAA+probe-aCnc
- 38180-6 - HCV RNA SerPl NAA+probe-Log IU
- 19147-8 - Ref Lab Test Ref Range
- 62365-2 - Diagnostic imp Spec-Imp
- 92731-9 - HCV Gentyp SerPl Seq
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
1.5 mL
Container
Gel-barrier tube or serum transfer tube
Collection Instructions
Two samples must be submitted. If a tube other than a gel-barrier tube is used, transfer separated serum to a plastic transport tube. Centrifuge sample within 24 hours of collection. Ship frozen (preferred).
Patient Preparation
Patients should stop biotin consumption at least 72 hours prior to the collection of a sample.
Storage Instructions
Antibody test: refrigerated (preferred), PCR test: frozen (preferred)
Causes for Rejection
Incorrect anticoagulant; PPT not centrifuged; frozen gel-barrier tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Antibody test: 14 days, PCR test: 24 hours |
| Refrigerated | Antibody test: 14 days, PCR test: 72 hours |
| Frozen | Antibody test: 14 days, PCR test: 6 weeks |
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