Hepatitis C Virus (HCV) Antibody Cascade to Quantitative PCR and Genotyping
Use
Diagnosis and management of hepatitis C virus (HCV) infection. A positive antibody test will reflex to a quantitative PCR assay. When the quantitative viral load is >1000 IU/ml an additional reflex to genotyping will occur.
Special Instructions
Submit two samples, preferably one refrigerated unopened gel-barrier tube and one frozen serum/plasma pour off tube. It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Limitations
The test may exhibit interference when a sample is collected from a person who is consuming a supplement with a high dose of biotin. Additionally, results may vary in accuracy in immunocompromised individuals where early or acute infection might delay detection.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 40726-2
- 13955-0
- 11011-4
- 38180-6
- 19147-8
- 62365-2
- 92731-9
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
1.5 mL
Container
Gel-barrier tube or serum transfer tube
Collection Instructions
Two samples must be submitted. If a tube other than a gel-barrier tube is used, transfer separated serum to a plastic transport tube. Centrifuge sample within 24 hours of collection. Ship frozen (preferred).
Patient Preparation
Patients should stop biotin consumption at least 72 hours prior to the collection of a sample.
Storage Instructions
Antibody test: refrigerated (preferred), PCR test: frozen (preferred)
Causes for Rejection
Incorrect anticoagulant; PPT not centrifuged; frozen gel-barrier tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Antibody test: 14 days, PCR test: 24 hours |
| Refrigerated | Antibody test: 14 days, PCR test: 72 hours |
| Frozen | Antibody test: 14 days, PCR test: 6 weeks |