HER-2/neu FISH, Gastric, Paraffin
Also known as: Gastric Biopsy
Use
Qualitative determination of HER-2/neu gene amplification; prognostic information regarding risk of recurrence and disease-related death; predict response to therapies, including targeted immunotherapy
Special Instructions
Direct any questions regarding this test to oncology customer service at 800-345-4363.
Limitations
The use of fixatives other than 10% formalin and fixation times outside of six to 48 hours may not yield reliable results. Results of the HER-2/neu FISH assay should be used in conjunction with other information from the clinical evaluation of the patient and other prognostic factors, including tumor size, histologic grade, nodal status, patient's age, hormone receptor status, and other known risk factors.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 31150-6
- 31150-6
- 49683-6
- 74860-8
- 74861-6
- 49549-9
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
One paraffin block or four unstained, positively charged tissue slides cut at 4 to 5 microns and dried overnight at 56°C.
Minimum Volume
Not provided
Container
Paraffin block transport pouch or slide mailer.
Collection Instructions
FFPE tissue, preserved in 10% neutral buffered formalin, fixed for 18 to 24 hours. Extended fixation time might increase the incubation time required for pepsin digestion. ASCO/CAP 2013 guidelines recommend fixation time between 6 and 72 hours for breast tissue; however, special ASCO/CAP guidelines have not been established for gastric cancer samples.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Fixative other than neutral buffered formalin; quantity not sufficient for analysis; tumor other than gastric tumor