HER-2/neu Immunohistochemistry (IHC), Gastric Paraffin
Also known as: c-erbB-2
Use
HER-2-positive carcinomas predict response to humanized monoclonal antibody trastuzumab (Herceptin®) in conjunction with chemotherapy in patients with advanced or metastatic gastric or gastro-esphageal junction carcinoma.
Special Instructions
Not provided.
Limitations
Use of fixatives other than 10% formalin, fixation times less than six hours or greater than 48 hours may not yield reliable results.
Methodology
Immunoassay (IHC)
Biomarkers
HER-2/neu
Protein
LOINC Codes
- 18474-7 - Her2 Ag Tiss Ql ImStn
- 18474-7 - Her2 Ag Tiss Ql ImStn
- 77202-0 - Laboratory comment Report
- 69426-5 - Medical director review
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
One paraffin block or five unstained, positively charged tissue slides cut at 4 to 5 microns and dried for one hour at 60°C.
Minimum Volume
Not provided
Container
Paraffin block transport pouch or slide mailer
Collection Instructions
FFPE tissue, preserved in 10% neutral buffered formalin, fixed for 18 to 24 hours. ASCO/CAP 2013 guidelines recommend fixation time between six and 72 hours for breast tissue; however, special ASCO/CAP guidelines have not been established for gastric cancer samples.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Tumor other than gastric tumor
Other tests from different labs that may be relevant