HLA Class I Antibody C1q Binding Assay
Also known as: C1q Fixing Class 1 Human Leukocyte Antigen Antibodies, Complement Fixing Antibody to Class 1 HLA Antigens
Use
The HLA Class I Antibody C1q Binding Assay is used to detect the presence of complement-fixing HLA Class I antibodies. These antibodies have clinical significance in the context of organ transplantation, as they can be indicative of humoral rejection or a higher risk of graft dysfunction. The C1q binding capability of these antibodies can help differentiate between potentially harmful and non-harmful antibodies, providing valuable insights into the management and risk stratification of transplant patients.
Special Instructions
Not provided.
Limitations
The assay specifically detects complement-fixing HLA Class I antibodies and does not provide information on non-complement fixing antibodies. False positives or negatives may occur due to interfering substances or incorrect specimen handling. Hemolysis or use of the wrong collection tube can lead to specimen rejection. Interpretation should consider clinical history and other diagnostic tests.
Methodology
Other
Biomarkers
HLA Class I C1q
Protein
LOINC Codes
- 46994-0 - HLA-A+B+C Ab NFr Ser
- 46994-0 - HLA-A+B+C Ab NFr Ser
- 49549-9 - Ref lab test method
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
7 mL
Minimum Volume
3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Patient Preparation
None
Storage Instructions
Refrigerate.
Causes for Rejection
Hemolysis; wrong collection tube
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days (stability determined by manufacturer or literature reference) |
| Frozen | Up to 11 months (stability determined by manufacturer or literature reference) |
Other tests from different labs that may be relevant