Human Immunodeficiency Virus 1 (HIV-1) PhenoSense Integrase® (Monogram® Integrase Inhibitor Resistance)
Also known as: PhenoSense Integrase®, Raltegravir
Use
The PhenoSense Integrase® assay is a phenotypic resistance test used to determine the susceptibility of a patient's HIV to integrase inhibitors. Integrase inhibitors block HIV replication by preventing the virus from integrating into the host cell's DNA. Raltegravir (Isentress®) is the first approved drug in this class of HIV antiretroviral drugs.
Special Instructions
Collect specimen in two lavender-top (EDTA) tubes or two PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection and transfer plasma to one or more polypropylene screw-capped tube(s) and freeze. Ship specimen frozen. Submit separate frozen specimens for each test requested to avoid delays in turnaround time.
Limitations
The PhenoSense Integrase® test should be used for patients with documented HIV-1 infection with viral loads ≥500 copies/mL. It should be interpreted alongside other clinical and laboratory findings and not be used as the sole criterion for patient management. Additionally, interactions between protease-reverse transcriptase (PR-RT) and integrase may impact the complete virus fitness but are not well-characterized, indicating a potential limitation in the context of comprehensive viral fitness assessment.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 45182-3
- 61199-6
- 77202-0
- 45182-3
- 61199-6
- 77202-0
Result Turnaround Time
19-23 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3 mL
Minimum Volume
Not provided
Container
Lavender-top (EDTA) or plasma preparation tube (PPT™)
Collection Instructions
Collect specimen in two lavender-top (EDTA) tubes or two PPT™ tubes. Centrifuge within six hours of collection, transfer plasma to polypropylene screw-capped tubes, and freeze. Ship frozen.
Storage Instructions
Freeze
Causes for Rejection
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens