KRAS Gene Mutation Analysis, Extended
Use
KRAS is a guanosine triphosphate (GTP) binding protein involved in downstream receptor signaling, which is critical for cell proliferation, survival, and differentiation. Mutations in the KRAS oncogene are frequently found in human cancers. They are common in pancreatic, colorectal, lung, gallbladder, bile duct, and thyroid cancer. This assay will detect KRAS mutations in exons 2, 3, and 4, allowing the determination of drug response.
Special Instructions
Not provided.
Limitations
This assay is able to detect 5% mutation in a background of wild-type DNA.
Methodology
SNaPshot Multiplex PCR (primer extension-based method)
Biomarkers
LOINC Codes
- 21702-6 - KRAS gene Mut Anl Bld/T
- 42349-1
- 31208-2 - Specimen source
- 57723-9 - Unique bar code # Current sample
- 48004-6 - DNA change
- 48005-3 - Amino acid change
- 72486-4 - Lab director name Provider
- 21703-4 - KRAS gene Mut Tested Bld/T
- 49549-9 - Ref lab test method
- 75608-0 - Citation Ref Lab Test
- 8100-0 - Specimen preparation
- 18781-5
- 39111-0 - Bdy site
- 55752-0 - Clinical info
- 62365-2 - Diagnostic imp Spec-Imp
- 77202-0 - Laboratory comment Report
- 19139-5 - Pathologist name
- 51969-4 - Gene analysis narr rpt Doc
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Unknown
Volume
Not provided
Minimum Volume
Not provided
Causes for Rejection
Tumor block containing no tumor tissue; broken or stained slides
Other tests from different labs that may be relevant