Lipoprotein Phenotyping Profile
Use
Evaluate hyperlipidemia to determine abnormal lipoprotein distribution and concentration in the serum.
Special Instructions
State patient's age, sex, and pertinent clinical history on the test request form. Ensure patient is fasting for 12 to 14 hours before specimen collection to obtain accurate results.
Limitations
The test can be affected by non-fasting states, leading to misclassification of electrophoretic patterns due to food intake. Drugs acting as delipidizing agents can also produce misleading patterns. False results may arise in conditions like dysglobulinemia where lipid is bound to immunoglobulins, necessitating confirmation or exclusion via agarose gel protein electrophoresis. The PHLA measurement can confirm Type I hyperlipoproteinemia, which distinguishes it from Type V based on PHLA activity levels.
Methodology
Other
Biomarkers
LOINC Codes
- 91136-2
- 2093-3
- 2571-8
- 2085-9
- 13458-5
- 13457-7
- 8251-1
- 20510-4
Result Turnaround Time
5-9 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
1 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within 45 minutes of collection. Lipid panels are best avoided following acute myocardial infarct, for up to three months, although cholesterol can be measured in the first 24 hours.
Patient Preparation
Patient should be on stable diet, ideally for two to three weeks prior to collection of blood, and should fast for 12 to 14 hours before collection of the specimen.
Storage Instructions
Refrigerate
Causes for Rejection
Hemolysis; patient not fasting; heparinized plasma; room temperature specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unstable |
| Refrigerated | 14 days |
| Frozen | 14 days |