Acid-Fast Smear for Mycobacterium, Varies
Use
This test is used for the detection of acid-fast bacilli in clinical samples. It helps in the rapid identification of individuals who are likely to be infected with mycobacteria, particularly Mycobacterium tuberculosis, which is a leading infectious disease cause of death worldwide. The Centers for Disease Control and Prevention has reported an increase in the incidence of tuberculosis associated with AIDS, foreign-born cases, and increased transmission in high-risk populations. The detection of acid-fast bacilli in sputum and other specimens allows for prompt identification of potentially infected persons, enabling timely diagnosis and isolation to prevent further spread.
Special Instructions
Not provided.
Limitations
The test may encounter artifacts that exhibit nonspecific fluorescence, which can be confused with actual organisms in the specimen. Swab specimens have generally low yield for mycobacteria recovery as compared to fresh tissue or body fluid specimens. Additionally, the detection is limited to the presence of acid-fast bacilli, and further molecular testing or culture may be needed for definitive diagnosis.
FDA ApprovedNew York Approved
Methodology
Other (Auramine-Rhodamine Stain)
Biomarkers
Mycobacterium tuberculosis
Microorganism
LOINC Codes
- 676-7 - Rhodamine-auramine Stn Spec
- 676-7 - Rhodamine-auramine Stn Spec
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Body Fluid
Volume
1 mL
Minimum Volume
Not provided
Container
Sterile container
Collection Instructions
Submit only 1 of the following specimens: Saliva is not acceptable.
Causes for Rejection
Specimen in viral transport medium, wood shaft, charcoal or gel swab.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |