Actin, Smooth Muscle (SMActin) Immunostain, Technical Component Only
Use
This test is useful for identifying cells expressing the alpha-smooth muscle isoform of actin. It involves labeling smooth muscle cells of vessels, myoepithelial cells, pericytes, some stromal cells in the intestine, testis, and ovary, and tumors derived from smooth muscle cells. It does not react with actin from fibroblasts, striated muscle, and myocardium, making it a useful tool in identifying leiomyomas, leiomyosarcomas, and pleomorphic adenomas.
Special Instructions
This test includes only the technical performance of the immunostain and does not provide pathologist interpretation. For interpretation, order a full pathology consultation. The positive and negative controls are verified for appropriate immunoreactivity.
Limitations
Age of a cut paraffin section can affect immunoreactivity, and stability thresholds are antigen-dependent. The charge of glass slides can be affected by environmental factors, potentially altering slide staining. Best practices include minimizing storage time after unpackaging, limiting exposure to humidity and heat, and avoiding plastics.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only; no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides per test ordered, sections 4-microns thick.
Storage Instructions
Ambient preferred, refrigerate if necessary. Avoid high humidity and heat exposure.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |