Acute Myeloid Leukemia (AML), Specified FISH, Varies
Use
The AML Specified FISH test is particularly useful for detecting recurrent chromosome abnormalities associated with acute myeloid leukemia (AML). It serves as an adjunct to conventional chromosome studies in patients with AML and is employed in evaluating specimens where chromosome studies are unsuccessful. The test is also utilized for identifying and tracking known chromosome abnormalities in AML patients, monitoring therapy response, and is not intended as a panel test. Specific probes must be specified in the order request field to ensure appropriate testing.
Special Instructions
This test is intended for cases where limited AML FISH probes are needed. Targeted probes must be specified on the ordering request. If not specified, test processing may be delayed and may result in test cancellation and auto-reordering as AMLFA or AMLFP, depending on patient age. Reflex probes can be performed if appropriate and specified in the request. Minimal residual disease monitoring should be done by PCR, and not by FISH.
Limitations
This test is not FDA-approved and is best used as an adjunct to clinical and pathologic information. It does not substitute for conventional chromosome studies that may detect other abnormalities undetectable by AML FISH. Bone marrow is the preferred specimen type, but if unavailable, blood with circulating myeloblasts may also be used. If no FISH signals are observed post-hybridization, results will indicate a lack of FISH results.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 102103-9
- 50397-9
- 69965-2
- 93356-4
- 62356-1
- 42349-1
- 78040-3
- 31208-2
- 85069-3
- 48767-8
- 62364-5
- 18771-6
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 to 3 mL
Minimum Volume
1 mL
Container
Yellow top (ACD), Green top (sodium heparin) or lavender top (EDTA)
Collection Instructions
Preferably send the first aspirate from the bone marrow collection. Invert several times to mix bone marrow. Send bone marrow in the original tube. Do not aliquot.
Causes for Rejection
Fresh tissue