Alpha-1-Antitrypsin Immunostain, Technical Component Only
Use
Alpha-1-antitrypsin (AAT) is a plasma protein synthesized in the liver and present in serum and tissue fluids where it acts as an inhibitor of proteases, especially elastase. AAT deficiency is associated with development of emphysema and liver disease. In liver disease, abnormal accumulation of AAT is seen as cytoplasmic globules in hepatocytes. This test is useful for identifying abnormal accumulation of alpha-1-antitrypsin.
Special Instructions
This test includes only technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Any immunohistochemistry (IHC)/in situ hybridization (ISH) stain performed as part of the PATHC will be performed at the reviewing pathologist's discretion at an additional charge. Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Limitations
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. The positive and negative controls are verified as showing appropriate immunoreactivity. Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |