Alpha-Amylase, IgE, Serum
Use
The Alpha-Amylase, IgE test is useful for establishing the diagnosis of an allergy to Alpha-Amylase and defining the specific allergen responsible for eliciting allergic signs and symptoms. It helps in identifying allergens responsible for allergic responses and/or anaphylactic episodes, and can confirm sensitization prior to the beginning of immunotherapy. The test is crucial in investigating the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens. It is important to note that testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists or in patients for whom the medical management does not depend upon identification of allergen specificity.
Special Instructions
Not provided.
Limitations
The test is not useful for patients who have undergone immunotherapy or for those where the management does not rely on specific allergen identification. Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies, leading to potential false positives. Moreover, individuals with markedly elevated serum IgE (>2500 kU/L) may exhibit false-positive results due to nonspecific binding. Hence, test results must be interpreted in the clinical context.
FDA ApprovedNew York Approved
Methodology
Immunoassay (FEIA)
Biomarkers
LOINC Codes
- 7073-0 - Amylase IgE Qn
- 7073-0 - Amylase IgE Qn
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL for every 5 allergens
Minimum Volume
For one allergen: 0.3 mL, for more than one allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis: OK, Gross lipemia: OK
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |