Alpha Beta Double-Negative T Cells for Autoimmune Lymphoproliferative Syndrome, Blood
Use
The test is useful for diagnosing autoimmune lymphoproliferative syndrome (ALPS), primarily in patients younger than 45 years. Autoimmune lymphoproliferative syndrome is a complex clinical disorder characterized by lymphoproliferative disease, autoimmune cytopenias, splenomegaly, and lymphadenopathy with increased susceptibility to malignancy. Genetic defects in the apoptosis pathway play a key role in this condition.
Special Instructions
Not provided.
Limitations
The test is typically not indicated for older adults, as the sole presence of increased alpha beta T-cell receptor + double-negative T cells B220+ cells is not sufficient for a diagnosis of ALPS. Additional testing is required to confirm a diagnosis.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Alpha beta TCR+DNT
Cell Type
LOINC Codes
- 101414-1 - Lymphocyte TCR A B dbl neg sub pnl Bld
- 88053-4 - Cell.DNT-CD45R+TCRa b # Bld FC
- 34963-9 - CD3+TCR A-B+ Cells # Bld
- 88052-6 - Cells.DNT-CD45R+TCR A-B+/CD3 Bld FC
- 34962-1 - CD3+TCR A-B+ Cells NFr Bld
- 69052-9 - Flow cytometry specialist review
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
0.5 mL
Container
Lavender top (EDTA)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Patient Preparation
For serial monitoring, it is recommended that specimens are collected at the same time of day.
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |