Amyloid Precursor Protein (APP) Immunostain, Technical Component Only
Use
This test aids in the identification of amyloid precursor protein present in Alzheimer's disease. Amyloid precursor protein (APP) is present in Alzheimer disease-associated plaques, large pyramidal cells as well as smaller neurons, astrocytes, and microglia. Histologic features of Alzheimer disease include the presence of abundant neurofibrillary tangles, neuropil threads, and neuritic ('senile') plaques. The main component of senile plaque amyloid is a 39- to 42-amino acid segment referred to as beta amyloid, derived from APP.
Special Instructions
Not provided.
Limitations
This test does not include pathologist interpretation, only the technical performance of the stain. If interpretation is required, a PATHC / Pathology Consultation should be ordered for a full diagnostic evaluation or a second opinion of the case. Additionally, the age of a cut paraffin section can affect immunoreactivity, and stability thresholds vary widely depending on antigen dependency. It is best practice for paraffin sections to be cut within six weeks. Unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Amyloid Precursor Protein
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block OR 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Ambient (preferred); Refrigerated also acceptable.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides