Amyloid Protein Identification, Paraffin, Mass Spectrometry
Use
Amyloidosis is a hereditary and acquired diseases group characterized by extracellular deposition of misfolded proteins leading to end organ damage. Specific amyloid-related diseases are associated with specific amyloid proteins, such as kappa or lambda immunoglobulin light chains (AL amyloid), transthyretin (ATTR amyloid), and serum amyloid A (SAA amyloid). Accurate protein identification is crucial since treatment varies based on amyloid subtype. This test employs Congo red staining followed by laser microdissection and mass spectrometry to reliably determine the identity of proteins constituting the amyloid.
Special Instructions
Not provided.
Limitations
False-negative results may occur with Congo red staining. Cases not recognized as amyloid may require correlation with clinical and pathological features for definitive diagnosis. The test relies on laser microdissection and mass spectrometry, which requires Congo red-positive samples.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
Unknown protein
Protein
LOINC Codes
- 101405-9 - AMPIP/AMPIF Tiss Doc
- 50595-8 - Path Interp Spec-Imp
- 19139-5 - Pathologist name
- 81178-6 - Block description and site Spec
- 62364-5 - Test performance info Spec
- 80398-1 - Unique ID Current sample
Result Turnaround Time
7-15 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Do not send fixed tissue slides. Testing requires paraffin-embedded tissue blocks. If Congo red stain has been performed, send stained slide with tissue block.
Causes for Rejection
Fixed tissue slides, wet/frozen tissue, cytological smears, nonformalin fixed tissue, nonparaffin embedded tissue
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |