Anti-DNase B Titer, Serum
Use
The Anti-DNase B Titer test is useful for demonstrating acute or recent streptococcal infection. Group A streptococci possess a variety of extracellular enzymatic proteins, including deoxyribonucleases (DNases A, B, C, and D). Infections by these bacteria can lead to complications such as rheumatic fever and glomerulonephritis. Diagnosing a recent streptococcal infection through serological assays like the anti-DNase B titer helps in understanding these conditions, especially when skin infections are implicated, as they are associated with a poor ASO response.
Special Instructions
Not provided.
Limitations
False-high titers can occur in patients with liver disease, where high lipoprotein concentrations in the serum mimic antibody activity. Additionally, sera with turbidity or significant light scattering properties due to lipemia may lead to erroneous quantitation results by the nephelometric method. Proper specimen handling and transportation are essential to avoid contamination by bacterial organisms that could alter titers.
New York Approved
Methodology
Immunoassay (Nephelometry)
Biomarkers
Anti-DNase B
Protein
LOINC Codes
- 5133-4 - Strep DNAse B Ser-aCnc
- 5133-4 - Strep DNAse B Ser-aCnc
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Patient Preparation
Fasting: 8 hours, preferred but not required
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 28 days |
| Frozen | 28 days |