Aspergillus Antigen, Bronchoalveolar Lavage
Use
The Aspergillus Antigen test using bronchoalveolar lavage (BAL) specimens is useful for aiding in the diagnosis of invasive aspergillosis (IA), a severe infection in patients with prolonged neutropenia after transplantation or undergoing aggressive immunosuppressive therapies. These patients are at high risk due to the rapid progression of IA and its high mortality rate. The test detects galactomannan, a molecule found in the cell wall of Aspergillus species, thereby enabling earlier diagnosis and potential initiation of antifungal therapy before the infection becomes life-threatening.
Special Instructions
For serum specimens, a different test should be ordered (ASPAG / Aspergillus (Galactomannan) Antigen, Serum). Use only sterile, leak-proof containers for specimen collection to avoid rejection. Complete the Infectious Disease Serology Test Request form if not ordering electronically.
Limitations
There is a noteworthy rate of false-positive results (8%-14%) in serum due to cross-reactivity with other substances or species. Other fungal species like Penicillium and Paecilomyces may also cause reactivity, potentially affecting the specificity for Aspergillus. False positives have been associated with PLASMA-LYTE solutions or fungal digestive enzymes. The performance of the test has not been assessed for specimen types such as urine or cerebrospinal fluid, and its sensitivity may be reduced by concurrent antifungal therapy or in patients with chronic granulomatous disease or hyper-IgE syndrome. Precautions in specimen handling are essential for avoiding contamination.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 62467-6
- 62467-6
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Body Fluid
Volume
2 mL
Minimum Volume
1.5 mL
Container
Sterile, leak-proof container
Collection Instructions
Avoid using specimen trap collection containers with suction catheters. If necessary, transfer specimens in a biosafety cabinet.
Storage Instructions
Refrigerate specimens immediately if transport will be delayed, and place them in a separate sealed plastic bag.
Causes for Rejection
Specimen trap collection containers, bronchial washings, thick/viscous/mucoid specimens, specimens in non-leak proof containers.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 14 days |