Bartonella, Molecular Detection, PCR, Varies
Use
Aiding in the diagnosis of Bartonella infection, which can cause conditions like cat scratch disease, bacillary angiomatosis, peliosis hepatitis, and endocarditis. The test uses PCR to detect Bartonella species by amplifying a specific sequence of the citrate synthase gene. This real-time PCR test provides rapid identification and is more sensitive than culture, which has limitations due to Bartonella's fastidious growth requirements.
Special Instructions
Specimen source is required when submitting samples. For electronic orders, ensure the Microbiology Test Request form accompanies the specimen if not ordering electronically. If Bartonella infection is strongly suspected despite a negative PCR result, consider alternative tests such as Bartonella Antibody Panel, IgG and IgM, and Warthin-Starry tissue stain.
Limitations
This test does not differentiate between Bartonella henselae and Bartonella quintana. A negative result does not rule out the presence of the organism or active disease due to potential PCR inhibition, sequence variability, or low DNA quantities. Inhibition has been noted in formalin-fixed, paraffin-embedded tissues. Results should be correlated with clinical presentation.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 48864-3
- 48864-3
- 31208-2
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Entire collection or 5 mm(3)
Minimum Volume
5 mm(3)
Container
Sterile container
Collection Instructions
Collect fresh tissue specimen. Submit tissue only, do not add fluid to tissue. Refrigerate or freeze specimen.
Storage Instructions
Refrigerated (preferred) 7 days / Frozen 7 days
Causes for Rejection
Tissue in formalin, formaldehyde, or acetone. Specimens other than those listed in Specimen Required. Slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 7 days |