Biotin, Serum
Use
This test is useful for the measurement of biotin in serum, assessment of biotin concentrations in individuals taking biotin supplements, and investigation of unexpected results from immunoassays that utilize biotin-streptavidin detection methods. It helps determine if a patient's high biotin concentrations are likely from biotin supplementation or treatment. Elevated biotin levels may interfere with certain laboratory assays.
Special Instructions
If testing for biotinidase deficiency is requested, order BIOTS / Biotinidase, Serum. Serum biotin is typically found at low endogenous concentrations but can be elevated due to supplementation. Freeze specimens within 3 days of collection and send frozen.
Limitations
The test is not useful as a screen for biotinidase deficiency. It is unknown how long biotin will remain elevated in patients taking supplements, as this varies by dose, duration of supplementation, and time of blood draw post-ingestion. High levels of serum biotin can interfere with some laboratory assays that use biotin-streptavidin chemistry.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 1980-2
- 1980-2
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Storage Instructions
Freeze specimens within 3 days of collection and send frozen.
Causes for Rejection
Gross hemolysis: OK; Gross lipemia: OK; Gross icterus: OK
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 7 days |
| Frozen | 30 days |