BK Virus DNA Detection and Quantification, Plasma
Use
This test is useful for the detection and serial monitoring of BK virus-associated nephropathy in kidney transplant recipients using plasma specimens. It assists in monitoring BK virus-associated hemorrhagic cystitis in organ transplant recipients. The assay quantifies the level of BK virus (BKV) DNA present in the plasma, which is critical for managing patients at risk of developing BKV-associated nephropathy leading to decreased kidney function and eventual failure.
Special Instructions
The assay is calibrated to the First World Health Organization International Standard for BKV DNA. It is recommended to centrifuge blood collection tube and aliquot plasma into a plastic vial immediately. If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80 degrees C (up to 84 days) until shipment on dry ice.
Limitations
On average, quantitative BK virus (BKV) DNA results in plasma tested with this assay can be up to 1.4-fold (about 0.15 log IU/mL) higher than those generated from the previous methodology due to differences in extraction method and design in amplification primers and probes. A single 'Undetected' test result does not necessarily rule out the presence of BKV infection. Serial measurement is recommended for monitoring BKV replication status.
New York Approved
Methodology
PCR-based (Real-Time Polymerase Chain Reaction (PCR))
Biomarkers
LOINC Codes
- 32284-2
- 32284-2
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge blood collection tube and aliquot into a plastic vial per manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes). Freeze aliquoted plasma for shipment.
Storage Instructions
Ship specimen frozen on dry ice only. If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80 degrees C (up to 84 days) until shipment on dry ice.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days |
| Frozen | 84 days |