Broccoli, IgE, Serum
Use
This test is used for establishing a diagnosis of an allergy to broccoli. It helps define the allergen responsible for causing allergic reactions and is useful in identifying allergens that contribute to allergic responses and/or anaphylactic episodes. Additionally, it confirms sensitization prior to immunotherapy and investigates specific allergic reactions to various allergens including insect venom, drugs, or chemicals. Testing for IgE antibodies is not recommended for patients who have undergone immunotherapy to determine residual sensitivity, or when allergen specificity does not impact medical management.
Special Instructions
Care should be taken when interpreting results for patients with clinically insignificant sensitivities that may have measurable IgE levels. False positives may occur in patients with significantly elevated serum IgE (>2500 kU/L) due to nonspecific binding.
Limitations
Testing for IgE antibodies is not beneficial for patients previously treated with immunotherapy for assessing residual clinical sensitivity. The test might not be useful if the identification of allergen specificity does not guide medical management. False positives can occur in patients with high serum IgE levels due to nonspecific binding.
FDA ApprovedNew York Approved
Methodology
Immunoassay (Fluorescence Enzyme Immunoassay (FEIA))
Biomarkers
LOINC Codes
- 6052-5
- 6052-5
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL for every 5 allergens requested
Minimum Volume
0.3 mL for 1 allergen; 0.05 mL per additional allergen plus 0.25 mL deadspace
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis and gross lipemia are acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |