Bupropion and Metabolite, Serum
Use
This test is used to measure the serum concentrations of bupropion and its major metabolite, hydroxybupropion. Monitoring these concentrations is important because bupropion is used as an antidepressant and to help with smoking cessation, marketed under the names Wellbutrin and Zyban, respectively. Determining the concentration of bupropion can help assess therapeutic levels and adjust doses accordingly. It is known that maximum antidepressant response occurs at trough plasma concentrations between 50 to 100 ng/mL, with significantly reduced therapeutic response below 25 ng/mL.
Special Instructions
Specimens must be submitted in a Red top collection tube; serum gel/SST tubes are not acceptable. The specimen must be refrigerated after centrifuging and aliquoting into a plastic vial for transport.
Limitations
The test has not been cleared or approved by the U.S. Food and Drug Administration. It was developed and its performance characteristics determined by NMS Labs. Specimens must be kept frozen to prevent lower or negative value results, which may occur if the specimens are not properly stored.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 6706-6
- 9418-5
Result Turnaround Time
6-10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot 1 mL of serum into a plastic vial. Send refrigerated.
Causes for Rejection
Gross hemolysis, polymer gel separation tube (SST or PST)
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 30 days |