Caldesmon Immunostain, Technical Component Only
Use
Caldesmon is a smooth muscle specific protein that regulates smooth muscle contraction. This clone recognizes the high-molecular-weight variant (h-caldesmon) and does not react with the nonmuscle variant. Neither variant of caldesmon is present in skeletal muscle. Anti-h-caldesmon seems to be a reliable marker of smooth muscle differentiation and may assist in the diagnosis of smooth muscle tumors.
Special Instructions
This test includes only technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled.
Limitations
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only; no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Use formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides. Sections should be 4-microns thick.
Storage Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides