Carbapenem Resistance Genes, Molecular Detection, PCR
Use
This test is used for detecting and differentiating blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP gene sequences that are associated with carbapenem intermediate or resistant results. It assists in infection control by detecting gastrointestinal colonization in healthcare settings of bacteria that are not susceptible to carbapenems using bacterial isolates from rectal or perirectal swabs. The assay targets specific carbapenemase genes to indicate potential resistance, aiding overall antibiotic stewardship.
Special Instructions
Not provided.
Limitations
The assay does not detect carbapenem resistance mechanisms other than blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP, including other carbapenemase types, porin mutations, or efflux pump hyperexpression. It is not suitable for bacterial identification, and detection of gene sequences does not necessarily indicate viable organisms. Variants or alterations in primer or probe binding regions could lead to false-negative results. The assay was not evaluated for carbapenem-resistant anaerobes or other OXA-carbapenemase genes except blaOXA-48 and blaOXA-181.
FDA ApprovedNew York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 85502-3 - BCR gene pnl Islt/Spm
- 85498-4 - blaIMP Islt/Spm Ql
- 85501-5 - blaVIM Islt/Spm Ql
- 73982-1 - blaNDM Islt/Spm Ql
- 49617-4 - blaKPC Islt/Spm Ql
- 85503-1 - blaOXA-48 Islt/Spm Ql
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Isolate
Minimum Volume
Not provided
Container
Slant
Collection Instructions
Perform isolation of infecting bacteria. Submit bacterial organism in pure culture, actively growing. Do not submit mixed cultures.
Causes for Rejection
Agar plate, Mixed culture
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |