Carbapenem Resistance Genes, Molecular Detection, PCR, Rectal Swab
Use
This test is useful for detecting and differentiating the bla_KPC, bla_NDM, bla_VIM, bla_OXA-48, and bla_IMP gene sequences associated with carbapenem intermediate or resistant results. It aids in infection control by detecting gastrointestinal colonization of patients in healthcare settings with bacteria not susceptible to carbapenems using rectal or perirectal swabs.
Special Instructions
Not provided.
Limitations
This test does not detect other mechanisms of carbapenem resistance, including carbapenemases not targeted by this assay, porin mutations, and hyperexpression of drug efflux pumps. It is not for bacterial identification or genetic-relatedness subtyping. The assay does not evaluate variants or alterations in primer or probe binding regions, which may affect detection. The detection of OXA-carbapenemase genes, apart from bla_OXA-48 and bla_OXA-181, has not been evaluated with this assay. Co-colonization with multiple carbapenemase-producing organisms is rare. Carbapenem-resistant anaerobes present in fecal specimens have not been evaluated by the Xpert Carba-R Assay.
FDA ApprovedNew York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 85502-3 - BCR gene pnl Islt/Spm
- 85498-4 - blaIMP Islt/Spm Ql
- 85501-5 - blaVIM Islt/Spm Ql
- 73982-1 - blaNDM Islt/Spm Ql
- 49617-4 - blaKPC Islt/Spm Ql
- 85503-1 - blaOXA-48 Islt/Spm Ql
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Entire collection
Minimum Volume
Not provided
Container
Culture transport swab
Causes for Rejection
Gross lipemia, Incorrect swab used
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 5 days |