CD45 Leukocyte Common Antigen (LCA) Immunostain, Technical Component Only
Use
CD45 is crucial for distinguishing lymphoma and leukemia from other neoplasms due to its high specificity and sensitivity for cells of hematolymphoid lineage. It is primarily used in scenarios where differentiation of hematolymphoid disorders is necessary. However, it should be noted that in classical Hodgkin lymphoma, CD45 expression is typically absent, making it an exception.
Special Instructions
Not provided.
Limitations
The test does not provide pathologist interpretation, and findings should be contextualized within the patient's clinical history by a qualified pathologist. Variations in glass slide charges caused by environmental factors can affect staining. It's necessary to minimize the storage time of positively charged slides and limit their exposure to high humidity and heat.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
CD45
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Best practices for storage of positively charged slides include minimization of time stored after being unpackaged and limiting exposure to high humidity and heat.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |