CD8 T-Cell Immune Competence, Global, Blood
Use
This test is useful for determining overimmunosuppression within the CD8 T-cell compartment. It is especially applicable for transplant recipients and patients with autoimmune disorders who are receiving therapy with immunosuppressant agents. Monitoring CD8 T-cell function helps in assessing the immunocompetence of individuals under immunosuppressive treatment and can guide the adjustment of therapeutic regimens to avoid overimmunosuppression, which can increase the risk of infections.
Special Instructions
Not provided.
Limitations
This assay is specific only for CD8 T cells and does not provide information for overall T-cell competence. Timing and consistency in timing of blood collection is critical when serially monitoring patients for lymphocyte subsets. Further studies are needed to determine if certain levels of Interferon-gamma and CD107a/b expression within the reference range confer varying degrees of infectious disease risk.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
CD107a/b
ProteinInterferon-gamma (IFN-gamma)
Protein
LOINC Codes
- 80222-3 - CD8 Cells/lymph NFr Spec
- 95203-6 - CD107ab CD8/CD3+CD8+ NFr Bld FC
- 95204-4 - IFN-g CD8 Cells/CD3+CD8+ Cll NFr Bld FC
- 69052-9 - Flow cytometry specialist review
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
15 mL
Minimum Volume
10 mL
Container
Green top (sodium heparin)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Storage Instructions
Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668).
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |