Chestnut, Sweet, IgE, Serum
Use
This test is used to establish a diagnosis of an allergy to sweet chestnut by detecting IgE antibodies in serum. It helps define the allergen responsible for eliciting allergic signs and symptoms and confirms sensitization to allergens prior to initiating immunotherapy. Additionally, it is used in the investigation of allergic responses to insect venom, drugs, or chemical allergens.
Special Instructions
For 1 allergen request, a minimum of 0.3 mL serum is required. For more than one allergen, a formula of (0.05 mL x number of allergens) + 0.25 mL should be applied to fulfill the deadspace requirement. Centrifuge and aliquot serum into a plastic vial and submit the specimen in a plastic vial. If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.
Limitations
Testing for IgE antibodies is not useful in patients already undergoing immunotherapy to determine residual clinical sensitivity. False positives for IgE antibodies may occur in patients with markedly elevated serum IgE levels (>2500 kU/L) due to non-specific binding to allergen solid phases. Test results need interpretation within the broader clinical context as individuals with clinically insignificant sensitivity may still show measurable levels of IgE antibodies.
FDA ApprovedNew York Approved
Methodology
Immunoassay (Fluorescence Enzyme Immunoassay (FEIA))
Biomarkers
LOINC Codes
- 6259-6
- 6259-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis: OK; Gross lipemia: OK
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 90 days |