Coccidioides immitis/posadasii, Molecular Detection, PCR, Varies

Casandra

Casandra Test Code MC36745Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID CIMRPCPT Code 87798

Coccidioides immitis/posadasii, Molecular Detection, PCR, Varies

Clinical Use

Use

This test is useful for the rapid detection of Coccidioides DNA and serves as an aid in diagnosing coccidioidomycosis. Coccidioidomycosis is caused by the dimorphic fungi, Coccidioides immitis and Coccidioides posadasii, which are endemic to the southwestern regions of the United States, northern Mexico, and areas of Central and South America. The PCR method allows for the identification of Coccidioides species directly from clinical specimens, enabling a rapid diagnosis. While culture remains the gold standard due to its high sensitivity, it is slower and presents safety hazards in the lab environment. Therefore, PCR provides a valuable, faster alternative for detecting these organisms.

Special Instructions

Specimen source must be indicated, and the specimen must arrive within 7 days of collection. For non-electronic orders, complete and send the Microbiology Test Request (T244) with the specimen. It is advised to perform this test in conjunction with fungal culture for comprehensive evaluation.

Limitations

A negative result does not conclusively rule out the presence of Coccidioides or active disease, as the organism may be present at levels below the assay's detection limit. The test cannot distinguish between viable organisms and nucleic acid from non-viable or previous infections. Additionally, this test does not differentiate between Coccidioides immitis and Coccidioides posadasii. Results should be interpreted alongside clinical symptoms and other diagnostic procedures.

Test Details

New York Approved