Coccidioides immitis/posadasii, Molecular Detection, PCR, Varies
Use
This test is useful for the rapid detection of Coccidioides DNA and serves as an aid in diagnosing coccidioidomycosis. Coccidioidomycosis is caused by the dimorphic fungi, Coccidioides immitis and Coccidioides posadasii, which are endemic to the southwestern regions of the United States, northern Mexico, and areas of Central and South America. The PCR method allows for the identification of Coccidioides species directly from clinical specimens, enabling a rapid diagnosis. While culture remains the gold standard due to its high sensitivity, it is slower and presents safety hazards in the lab environment. Therefore, PCR provides a valuable, faster alternative for detecting these organisms.
Special Instructions
Not provided.
Limitations
A negative result does not conclusively rule out the presence of Coccidioides or active disease, as the organism may be present at levels below the assay's detection limit. The test cannot distinguish between viable organisms and nucleic acid from non-viable or previous infections. Additionally, this test does not differentiate between Coccidioides immitis and Coccidioides posadasii. Results should be interpreted alongside clinical symptoms and other diagnostic procedures.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
Coccidioides
Microorganism
LOINC Codes
- 97916-1 - Coccidioides DNA Spec Ql NAA+probe
- 31208-2 - Specimen source
- 97916-1 - Coccidioides DNA Spec Ql NAA+probe
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Body Fluid
Volume
1 mL
Minimum Volume
0.5 mL
Container
Sterile container
Causes for Rejection
Blood, Bone marrow, Specimen in anaerobe vial or viral transport medium, Feces, Swabs, Tissues in formalin fluid, Urine
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |