Complement C3, Serum
Use
The test for Complement C3 in serum is primarily useful for assessing disease activity in conditions like systemic lupus erythematosus and in investigating cases with undetectable total complement levels. Complement components like C3 are integral to the immune system's defense, participating in pathways that are activated either by immune complexes or bacterial polysaccharides. Patients with C3 deficiency, either congenital or acquired due to certain diseases, may suffer recurrent bacterial infections or display symptoms associated with acute and chronic conditions such as glomerulonephritis or systemic lupus erythematosus.
Special Instructions
The test uses a standard nephelometric method for detecting antigen-antibody complexes in serum samples, which is crucial for the accurate measurement of Complement C3 levels. It is essential to submit the proper forms if not ordering electronically, with specific instructions for specimen handling and transport.
Limitations
The accuracy of nephelometry can be affected by certain conditions of the sample, such as turbidity due to lipemia. Grossly lipemic sera can lead to erroneous results due to light scattering properties. Therefore, proper specimen handling and storage conditions must be adhered to in order to avoid diagnostic confusion and ensure accurate measurements. The stability of the specimen is crucial as improper conditions can lead to degradation and inaccurate results.
FDA ApprovedNew York Approved
Methodology
Immunoassay (Nephelometry)
Biomarkers
LOINC Codes
- 4485-9
- 4485-9
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Storage Instructions
Refrigerated (preferred): 7 days; Ambient: 72 hours; Frozen: 28 days.
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 7 days |
| Frozen | 28 days |