Cortisol, Mass Spectrometry, Serum
Use
The test is used for second-order testing when cortisol measurement by immunoassay gives inconsistent results with clinical symptoms, or if patients are taking synthetic steroids. It serves as an adjunct in diagnosing primary and secondary adrenal insufficiency as well as Cushing syndrome. It is not recommended for evaluating response to metyrapone.
Special Instructions
Preferred morning (8 a.m.) and afternoon (4 p.m.) specimens should be submitted. Provide the time of collection and ensure samples are properly centrifuged and aliquoted into a plastic vial.
Limitations
This test is not recommended for evaluating response to metyrapone. Conditions such as stress, alcoholism, depression, and medication can affect the diurnal variation. High estrogen levels in women can falsely elevate plasma cortisol levels without increasing bioactive cortisol.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 87429-7
- 2143-6
- 9813-7
- 9812-9
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.6 mL
Minimum Volume
0.25 mL
Container
Red top (serum gel/SST are not acceptable)
Collection Instructions
Collect samples at 8 a.m. or 4 p.m., include collection time, centrifuge and aliquot into a plastic vial.
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 28 days |
| Refrigerated | 28 days |
| Frozen | 28 days |