Cytoplasmic Neutrophil Antibodies, Serum
Use
Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides are characterized by a pauci-immune inflammation within the walls of small blood vessels. There are 3 specific diseases identified as ANCA-associated vasculitides: microscopic polyangiitis (MPA), granulomatosus with polyangiitis (GPA), and eosinophilic granulomatosus with polyangiitis (EGPA). The serological hallmark of these disorders is the presence of ANCA, which are antibodies that bind to cytoplasmic antigens found in the granules of neutrophils. Patients with GPA frequently have antibodies specific for proteinase 3 (PR3), while individuals with MPA or EGPA are more likely to have antibodies that bind to myeloperoxidase (MPO).
Special Instructions
Not provided.
Limitations
A positive result for proteinase 3 (PR3)-ANCA, myeloperoxidase (MPO)-ANCA, or ANCA by IIF is not diagnostic for any ANCA-associated vasculitis and must be interpreted in the clinical context of the patient. Negative results for PR3-ANCA, MPO-ANCA, and ANCA by indirect immunofluorescence (IIF) do not exclude the possibility of ANCA-associated vasculitis. Antibodies specific for antigens other than PR3 and MPO may lead to nuclear, perinuclear or cytoplasmic staining on ethanol-fixed neutrophils.
New York Approved
Methodology
Immunoassay (Indirect Immunofluorescence)
Biomarkers
Myeloperoxidase (MPO)
AntibodyProteinase 3 (PR3)
Antibody
LOINC Codes
- 87427-1 - ANCA Pnl Ser IF
- 17357-5 - p-ANCA Ser Ql IF
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.8 mL
Minimum Volume
0.4 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into plastic vial. Use Sarstedt Aliquot Tube, 5 mL as Supply.
Causes for Rejection
Gross hemolysis, Gross lipemia, Heat-treated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 21 days |
| Frozen | 21 days |