Delta-Like 3 Protein (SP347), Semi-Quantitative Immunohistochemistry, Manual, Tissue
Use
The test is useful for the diagnosis of small cell lung carcinoma (SCLC) and large cell neuroendocrine carcinoma (LCNEC), among other tumors. Delta-like 3 protein (DLL3) is an atypical Notch ligand induced by the neuroendocrine transcription factor, ASCL-1, and is expressed on the cell membrane and in the cytoplasm of tumor cells. This test provides semi-quantitative determination of DLL3 expression using immunohistochemistry (IHC).
Special Instructions
The test requires a pathology/diagnostic report and a brief history for interpretation. Pathology Packaging Kit (T554) is used for submission. One slide will be stained with hematoxylin and eosin and will be returned to the client. Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting it into the courier mailer. Best practices are to minimize the time slides are stored after being unpackaged and limit exposure to high humidity and heat.
Limitations
A minimum of 100 tumor cells is required for interpretation. The test has not been validated on tissue or cell blocks subjected to alternative fixatives or decalcification. Age of a cut paraffin section can affect immunoreactivity, and stability thresholds vary. The charge of glass slides can be affected by environmental factors, which may alter slide staining. Best practices for the storage of positively charged slides include minimizing time stored after unpackaging and limiting exposure to high humidity and heat. Wet/frozen tissue, decalcified paraffin embedded tissue, nonformalin-fixed tissue, nonparaffin embedded tissue, and noncharged slides are causes for rejection.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 59465-5
- 19139-5
- 81178-6
- 62364-5
- 80398-1
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin-fixed, paraffin-embedded tissue block or 3 unstained glass, "positively charged" slides with 4-microns formalin-fixed, paraffin-embedded tissue
Collection Instructions
Attach green "Attention Pathology" address label (T498) to the outside of transport container before putting it into courier mailer.
Storage Instructions
Best practices for the storage are to minimize time stored, limit exposure to high humidity and heat, and avoid exposure to plastics.
Causes for Rejection
Wet/frozen tissue, decalcified paraffin embedded tissue, nonformalin-fixed tissue, nonparaffin embedded tissue, and noncharged slides. ProbeOn slides and Snowcoat slides are also rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |