Dihydrorhodamine Flow Cytometric Phorbol Myristate Acetate Test, Blood
Use
This test is useful for evaluating chronic granulomatous disease (CGD), including both X-linked and autosomal recessive forms, and complete myeloperoxidase deficiency. It monitors chimerism and nicotinamide adenine dinucleotide phosphate oxidase (NOX) function post-hematopoietic cell transplantation, assesses residual NOX activity pre-transplant, identifies female carriers for X-linked CGD, and evaluates changes in lyonization with age in female carriers.
Special Instructions
Not provided.
Limitations
Specimens should be collected in sodium heparin and optimally tested within 24 hours of blood draw (stability is within 48 hours). The test may be normal or mildly impaired in patients deficient in NCF4 (p40phox). Severe glucose-6-phosphate dehydrogenase deficiency can mimic CGD and affect DHR responses. Hemolyzed specimens may interfere with the assay. Specimens with an absolute neutrophil count less than 200 will be rejected. Complete myeloperoxidase deficiency may yield false-positive results requiring differentiation by specific fluorescence patterns and recombinant human MPO addition tests.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
NADPH oxidase complex
Protein
LOINC Codes
- 98124-1 - Neut PMA stim DHR Pnl Patient FC
- 751-8 - Neutrophils # Bld Auto
- 85376-2 - Neut PMA DHR/Neut PMA NFr Bld FC
- 85374-7 - Neut.PMA stim.DHR MFI Bld FC
- 85369-7 - Neutrophils # Cont Bld FC
- 85377-0 - Neut PMA DHR/Neut PMA NFr Cont Bld FC
- 85375-4 - Neut.PMA stim.DHR MFI Cont Bld FC
- 69052-9 - Flow cytometry specialist review
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
1 mL
Container
Green top (sodium heparin)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Storage Instructions
Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668). It is recommended that specimens arrive within 24 hours of collection.
Causes for Rejection
Gross hemolysis, Gross lipemia, Absolute neutrophil count less than 200
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |