Dilute Russell's Viper Venom Time (DRVVT), with Reflex, Plasma
Use
The Dilute Russell's Viper Venom Time (DRVVT) test is used for detecting and confirming the presence of lupus anticoagulants (LA), as well as helping to distinguish LA from specific coagulation factor inhibitors or deficiencies. It can be particularly useful for identifying LA that do not prolong the activated partial thromboplastin time (APTT) and for evaluating unexplained prolongation of the APTT or prothrombin time clotting tests.
Special Instructions
Specimens should be centrifuged and plasma separated before freezing. Each coagulation assay requested should have its own vial. Double-centrifuged specimens are critical to avoid platelet contamination which may cause false results. Ship all specimens in the same container, following coagulation guidelines for specimen handling.
Limitations
The DRVVT test may not detect all lupus anticoagulants, and some may only be detectable by other tests such as Staclot LA or APTT. Anticoagulant therapy can result in false-positive results. Residual platelets can decrease sensitivity and specificity leading to false negatives. Excess heparin or inhibitors of factors V or VIII could cause false positives.
FDA ApprovedNew York Approved
Methodology
Other (Optical Clot-Based)
Biomarkers
LOINC Codes
- 15359-3
- 15359-3
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge, remove plasma, and centrifuge plasma again. Aliquot into a separate plastic vial, leaving 0.25 mL in the bottom of the centrifuged vial. Freeze plasma immediately (no longer than 4 hours after collection) at -20 degrees C or, ideally, -40 degrees C or below.
Storage Instructions
Freeze plasma immediately after collection. Ship frozen at -20 degrees C or ideally, -40 degrees C or below.
Causes for Rejection
Gross hemolysis, gross lipemia, gross icterus.
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 14 days |