Fetomaternal Bleed, Flow Cytometry, Blood
Use
The test is primarily used to determine the volume of fetal-to-maternal hemorrhage to recommend an appropriate dose of the Rh immune globulin. This can prevent the maternal immune response in Rh-negative mothers carrying an Rh-positive fetus. It is crucial for preventing hemolytic disease of the newborn.
Special Instructions
Not provided.
Limitations
Clinical conditions such as hereditary persistence of fetal hemoglobin and thalassemia may cause increased levels of fetal hemoglobin-containing red blood cells, potentially affecting the results. Handling issues, such as aliquoting, can adversely affect results due to differential RBC densities.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Fetal Hemoglobin (HbF)
Analyte
LOINC Codes
- 75308-7 - Fetal blood Vol Bld FC
- 48767-8 - Annotation comment Imp
- 55730-6 - Fetal blood Vol Bld
- 10331-7 - Rh Bld
- 55731-4 - Inject Rh Ig Dose Qn
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
6 mL
Minimum Volume
1 mL
Container
Lavender top (EDTA)
Collection Instructions
Fill evacuated tube as completely as possible. Do not centrifuge. Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot as aliquoting into or out of a sample tube can adversely affect test results.
Causes for Rejection
Gross hemolysis, Gross lipemia, Gross icterus, Clotted blood
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 5 days |
| Refrigerated | 5 days |