Fibroblast Growth Factor-23 (FGF23), In Situ Hybridization
Use
The Fibroblast Growth Factor-23 (FGF23) test is used for assessing the expression of fibroblast growth factor-23 in tissues. FGF23 is a phosphaturic hormone that acts on the proximal renal tubules to block phosphate reuptake, which is crucial for diagnosing conditions like phosphaturic mesenchymal tumors responsible for tumor-induced osteomalacia. This test helps identify the presence and accumulation of FGF23 in affected tissues, aiding in the management and treatment planning of related disorders.
Special Instructions
Not provided.
Limitations
The test is limited by the condition of tissue sections. The age of paraffin sections can significantly affect immunoreactivity, which varies among antigens. Slides must be positively charged, with improper slide preparation leading to poor staining results. Additionally, non-formalin fixed or non-paraffin embedded tissue is unsuitable, and cytology smears cannot be processed by this test.
New York Approved
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Fibroblast Growth Factor-23 (FGF23)
Protein
LOINC Codes
- 104259-7 - FGF-23 tiss Ql FISH
- 50595-8 - Path Interp Spec-Imp
- 19139-5 - Pathologist name
- 81178-6 - Block description and site Spec
- 62364-5 - Test performance info Spec
- 80398-1 - Unique ID Current sample
Result Turnaround Time
5-8 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin-fixed, paraffin-embedded tissue block or 5 unstained, positively charged glass slides
Collection Instructions
Cut slides to 4-microns thickness. Ensure slides are positively charged and use the Pathology Packaging Kit (T554) for sending samples.
Storage Instructions
Keep slides in minimal exposure to humidity and heat; store properly to maintain integrity before analysis.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides