Fibulin 3 Immunostain, Technical Component Only
Use
Immunohistochemical staining for fibulin 3 (FBLN3), also known as epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is useful in confirming amyloid type. Antibodies to FBLN3 stain the amyloid deposits in patients with FBLN3 amyloidosis. FBLN3 amyloidosis typically involves the venous walls in the gastrointestinal tract.
Special Instructions
Not provided.
Limitations
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Fibulin 3
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Ambient (preferred), Refrigerated is acceptable.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Until staining is complete. |
| Refrigerated | Until staining is complete. |