Glomerular Basement Membrane Antibodies, IgG, Serum
Use
This test is useful for evaluating patients with clinical features of anti-glomerular basement disease, including rapidly progressive glomerulonephritis or pulmonary hemorrhage. Anti-glomerular basement (GBM) disease is a rare autoimmune disease associated with the presence of anti-GBM antibodies, particularly of the IgG isotype, which bind to the non-collagenous domain 1 (NC1) of the alpha3 chain of type IV collagen. These antibodies can trigger complement activation, leading to vascular necrosis and damage to the glomerular basement membrane. Evaluating the presence of these antibodies is crucial for diagnosing anti-GBM disease, particularly in patients with compatible clinical symptoms.
Special Instructions
Not provided.
Limitations
A positive result must be interpreted in the clinical context as it is not solely diagnostic for anti-GBM disease. A negative result does not exclude the possibility of anti-GBM disease, especially in patients treated with immunosuppressants or plasmapheresis prior to testing. Diagnostic sensitivity and specificity are suggested by a pooled sensitivity of 93% and specificity of 97% across various immunoassay methods. Clinical interpretation should consider these parameters, and higher antibody titers may correlate with increased mortality without prompt treatment.
New York Approved
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
IgG
Protein
LOINC Codes
- 31254-6 - BM IgG Ser-aCnc
- 31254-6 - BM IgG Ser-aCnc
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.35 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 21 days |
| Frozen | 21 days |